2004 Feb;50(2):91-6. doi: 10.1139/w03-112. Indica strains are known for their relaxing and calming effects, often used for stress relief and relaxation. This can be adjusted in special cases, but it is easy to cause major issues outside of this range. For non-coding transcripts, the fewest number of transcripts to keep at least 80% of present non-coding splice junctions were retained. Zhao JS, Greer CW, Thiboutot S, Ampleman G, Hawari J. A dot-plot between PWK/PhJ and the C57BL/6J reference illustrates the disruption of co-linearity at the PWK/PhJ Nlrp1b2 and Nlrp1d alleles (Fig. In particular, the wild-derived strains represent a rich resource of novel target sites, resistance alleles, genes and isoforms not present in the reference strain, or indeed many classical strains. It's also good for growing outdoors because it has an average flowering time of 54-61 days, and it can reach heights of 60-80 cm (24 - 32 inches). Medical marijuana patients choose Headspace to help relieve symptoms of stress, pain and anxiety. WGE: a CRISPR database for genome engineering. These transMapped transcript alignments were evaluated by a series of binary classifiers that attempt to diagnose differences between the parent and target genome. 1950 187, 35383545 (2011). Sequential anaerobic-aerobic degradation of munitions waste. In AugustusCGP the same types of evidence can be incorporated for either a subset or all species/ strains. M.K.S. Remember, the information provided in this section is intended to serve as a general guide. The consensus gene sets contain over 20,000 protein coding genes and over 18,000 non-coding genes (Fig. This is the first report of RDX degradation by a homoacetogen growing autotrophically and extends the number of genera known to carry out this transformation. Boniotto, M. et al. Genet. We identify and characterize 2,567 regions on the current mouse reference genome exhibiting the greatest sequence diversity. EMBO Mol. Sci. 51, 436445 (2014). This plant takes 7-10 weeks to flower and is speculated to have its genetic origins from Jamaica. From the pure landraces up Nat. Prostate Cancer 1, 118124 (2002). 14). Nucleic Acids Res. As someone with anxiety while high this strain was very good and easy. 4) that appear to have arisen either through retrotransposition (~80%) or gene duplication events (~20%). The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys. e, Box plot of sequence divergence (%)for LTRs, LINEs, and SINEs within and outside of hSNP regions. We use cookies for certain features and to improve your experience. 13, (2017). Cristina Sisu, Paul Muir, Mark Gerstein, Jobran Chebib, Benjamin C. Jackson, Peter D. Keightley, Shweta Ramdas, Ayse Bilge Ozel, Jun Z. Li, Karen H. Y. Wong, Walfred Ma, Pui-Yan Kwok, Andrew P. Morgan, Jonathan J. Hughes, Fernando Pardo-Manuel de Villena, Arang Rhie, Shane A. McCarthy, Erich D. Jarvis, Bjarni V. Halldorsson, Hannes P. Eggertsson, Kari Stefansson, Alexandre Almeida, Stephen Nayfach, Robert D. Finn, Nature Genetics 3c). 7, e1000112 (2009). 73, 196200 (2012). Standard operating procedures are described in more details elsewhere79. Combinatorial effects of odorants on mouse behavior. Neuroanatomical data (Supplementary Table 16) were analyzed using Students two-tailed equal variance test. Neuroanatomical studies were performed blind with experimenters not knowing the genotype of the mouse, on three 16-week-old matched control male mice in C57BL/6N background and three 16-week-old homozygous knockout of Efcab3. Clamp the barrel, padded, upright in your bench vise. Cell Mol. Novel mtDNA haplotypes were identified in PWK/PhJ and NZO/HlLtJ. An official website of the United States government. We examined retrotransposon content in hSNP dense regions on GRCm38 compared to an estimated null distribution (one million simulations) and found a significant enrichment of both LTRs (empirical P<1107) and long interspersed nuclear elements (LINEs) (empirical P<1107) (Supplementary Tables 11 and 12). The assembly and alignment of a variety of haplotypes at loci heterogenous amongst the laboratory strains allows for analysis of regions previously not placed in the reference assembly. Comparative Annotation Toolkit (CAT) simultaneous clade and personal genome annotation. Ross DE, Marshall CW, Gulliver D, May HD, Norman RS. We used these genomes to improve the mouse reference genome, resulting in the completion of 10 new gene structures. Due to popularity, many cultivars of the same name have cropped up, leading to confusion and dispute over the original genetics and breeder. Scaffold break parameters are shown in Supplementary Table 20. Ideraabdullah, F. Y. et al. d, Representative sagittal brain images of matched controls (left) and Efcab3-like mutant (right), showing a larger cerebellum, enlarged lateral ventricle and increased size of the pontine nuclei (n=3, see Supplementary Fig. For the latter, CDS and intron hints were generated from the GENCODE VM8 protein-coding gene set for the reference strain. It has been suggested that Nlrp1c may be the causal family member mediating resistance; Nlrp1c can be amplified from cDNA from PWK/PhJ macrophages but not CAST/EiJ54. European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK, Jingtao Lilue,Anthony G. Doran,Ruth Bennett,Paul Flicek,Adam Frankish,Jane Loveland,David Thybert&Thomas M. Keane, Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK, Jingtao Lilue,Anthony G. Doran,Monica Abrudan,William Chow,Joanna Collins,Petr Danecek,Dirk-Dominik Dolle,Matt Dunn,Richard Durbin,Paul Flicek,Adam Frankish,Beiyuan Fu,James Gilbert,Jennifer Harrow,Kerstin Howe,Ximena Ibarra-Soria,Chris J. Lelliott,Darren W. Logan,Jane Loveland,Naomi Park,Sarah Pelan,Mike Quail,Lesley Shirley,Charles Steward,Mark Thomas,Glen Threadgold,James Torrance,Kim Wong,Jonathan Wood,Fengtang Yang,David J. Adams&Thomas M. Keane, Center for Biomolecular Science and Engineering, University of California Santa Cruz, Santa Cruz, CA, USA, Ian T. Fiddes,Joel Armstrong,Mark Diekhans,Dent Earl&Benedict Paten, Institut de Gntique et de Biologie Molculaire et Cellulaire, Centre National de la Recherche Scientifique UMR7104, Institut National de la Sant et de la Recherche Mdicale U964, Universit de Strasbourg, Illkirch, France, Centre des Sciences du Got et de lAlimentation, University of Bourgogne Franche-Comt, Dijon, France, The Jackson Laboratory, Bar Harbor, ME, USA, Department of Genetics, University of Cambridge, Cambridge, UK, Brain Research Institute, University of California, Los Angeles, CA, USA, Yale Computational Biology and Bioinformatics, Yale University, New Haven, CT, USA, Mark Gerstein,Paul Muir,Fabio C. P. Navarro&Cristina Sisu, OxFORD Asset Management, OxAM House, Oxford, UK, Department of Computer Science and Engineering, University of California San Diego, La Jolla, CA, USA, Department of Pathology, Immunology, and Laboratory Medicine, University of Florida, Gainesville, FL, USA, Genetics Institute, University College London, London, UK, Institute of Mathematics and Computer Science, University of Greifswald, Greifswald, Germany, Stefanie Nachtweide,Lars Romoth&Mario Stanke, Cancer Research UK Cambridge Institute, University of Cambridge, Robinson Way, Cambridge, UK, German Cancer Research Center (DKFZ), Division Signaling and Functional Genomics, Heidelberg, Germany, Department of Bioscience, Brunel University London, Uxbridge, UK, Departamento de Biologa Celular y Molecular, Facultad de Ciencias Biolgicas, Pontificia Universidad Catlica de Chile, Santiago, Chile, Earlham Institute, Norwich Research Park, Norwich, UK, School of Life Sciences, University of Nottingham, Nottingham, UK, You can also search for this author in J. Appl. It THC levels between 10%-21%. For the paired-end libraries, 13 g DNA was sheared to 1001,000 bp using a Covaris E210 or LE220 and size selected (350450 bp) using magnetic beads (Ampure XP). WebThe strain yields 10 to 15 ounces per plant outdoors. For CAST/EiJ, PWK/PhJ, and SPRET/EiJ, we further scaffolded the assemblies with Dovetail Genomics long-range libraries. The smooth taste makes for an enjoyable smoke, as you float into a mellow headspace. High will clog probes. Clipboard, Search History, and several other advanced features are temporarily unavailable. Mitochondrial genome (mtDNA) assemblies for 14 strains supported previously published sequences20, although a small number of high-quality novel sequence variants in AKR/J, BALB/cJ, C3H/HeJ, and LP/J conflicted with GenBank entries (Supplementary Table 4). The expected effects are a little bit like the effects of a good friend: relaxed, happy, euphoric, uplifted, creative, and focused. All the information available on AskGrowers is only for educational purposes. I fall straight asleep with this. 5) and accounted for ~49% of all hSNPs (Supplementary Table 9 and Supplementary Fig. AugustusPB transcripts that could not be confidently assigned to parent transcripts were discarded and not evaluated for novel contribution. Patierno, S. R. et al. Best marijuana strains for sex at a glance Best cannabis strains for sex, female & male arousal: Mimosa Cannabinoid profile: Sativa-dominant Hybrid. The top 5% of hSNP dense windows was identified and a shared density cut-off per 10 kb window calculated (equivalent to 71 hSNPs per 10 kb window). Clostridium geopurificans strain MJ1 sp. Weiner, J. The Schlafen (chromosome 11: 82.983.3 Mb) family of genes are reportedly involved in immune responses, cell differentiation, proliferation and growth, cancer invasion, and chemotherapy resistance. 36, 11331137 (2004). Learn about the influencers, brands, We heard you are cool but we just want to make sure, You aren't old enough to visit Leafy Rewards Please come back when you are 18, A Beginners Guide to Edible Dosage: How to Consume Cannabis Safely, The Best Cannabis Strains for Creativity: Get Inspired Today, 7 Surprising Hemp Uses: From Beauty to Food, The Fascinating History of Cannabis: From Ancient Times to Today, How to Make Cannabis-Infused Ice Cream: A Delicious and Simple Recipe, Tourists Guide to Cannabis: Finding Top Dispensaries, The Secret Origins of 420: Unraveling the Mysteries of Cannabis Cultures Biggest Day, Top 15 Cannabis Instagram Accounts to Follow in 2023. Our guide explores the relationship between cannabis and imagination, including how to use it to enhance your creative pursuits. Positive F0 mice were further bred with C57BL/6NTac mice. All samples were also systematically assessed for cellular ectopia (misplaced neurons). et al. Each strain has a unique haplotype of Nlrp1 members, highlighting the extensive sequence diversity at this locus across inbred mouse strains (Fig. Each of the three M. m. domesticus strains (C67BL/6J, NOD/ShiLtJ, and WSB/EiJ) carries a different combination of Nlrp1 family members; Nlrp1d1f are novel strain-specific alleles that were previously unknown. & Earl, P. L. Identification of wild-derived inbred mouse strains highly susceptible to monkeypox virus infection for use as small animal models. Rapid heart rate? Bruce Banner specifically, its #3 phenotype offers incredible levels of THC and packs a punch that could humble even the most seasoned veteran. Cell Microbiol. These genomes identified a large, previously unannotated, gene (Efcab3-like) encoding 5,874 amino acids. For every strain into our database you will be able to create an unique map for all known hybrids and crossings! We'll explore top-quality strains, knowledgeable budtenders, and more. By design, AugustusTMR restricts gene finding to regions that align to a reference gene, and thus is not able to predict genes missing in the reference annotation or genes in unaligned regions. 60, 403409 (1996). 1b and Supplementary Fig. Biol. Your reply has been sent for verification, 2023 AskGrowers.com. carried out the genome annotation. Mol. Calm. The yield indoors depends on the growing area and can be as much as 1 ounce per square foot. Injected embryos were briefly cultured and oviductal embryo transfer performed in 0.5 days postcoital pseudopregnant female recipients (CBA/C57BL/6J). b, Evolutionary history of Efcab3-like in vertebrates including genome structure and surrounding genes. Libido definitely improved. The windows were then ordered by density of SNP (lowest, 1 hSNP per 10 kb window, to highest). Immunol. HHS Vulnerability Disclosure, Help The thalamus was also larger by 19% (P=0.007). All of the wild-derived strains we sequenced contain full-length Nlrp1d and exhibit a similar disruption of co-linearity at these alleles relative to C57BL/6J (Supplementary Data 6). PLoS Genet. 2009 Feb;20(1):15-26. doi: 10.1007/s10532-008-9195-1. Here you can look for Cannabis Strains who have certain genetic characteristics. The strains mysterious background hasnt stopped it from becoming popular around the world, though. Parasitology 142(Suppl, 1), S108S119 (2015). 14, R22 (2013). In particular, the wild-derived strains represent a rich resource of novel target sites, resistance alleles, genes and isoforms not present in the reference strain, or indeed Headspace is a mindfulness app with an easy-to-use interface that takes the seriousness and intimidation out of meditation. The initial contigs were assembled from the paired-end sequencing reads using SGA v0.9.43 (see URLs)72. Methods Gener. This is because the THC in the Headspace strain binds to receptors in your brain that make you feel relaxed and euphoric. mSystems. Cannabinoids are chemical compounds found in cannabis that interact with the body's endocannabinoid system to produce various effects. 123, 237245 (2015). Assembly of Slfn for the three CC founder strains of wild-derived origin (CAST/EiJ, PWK/PhJ, and WSB/EiJ) showed, for the first time, extensive variation at this locus. Mutant Efcab3-like mice display anomalies in multiple brain regions, suggesting a possible role for this gene in the regulation of brain development. Bruce Banner is a powerful hybrid cross between Strawberry Diesel and Ghosts cut of OG Kush. Deep genome sequencing and variation analysis of 13 inbred mouse strains defines candidate phenotypic alleles, private variation and homozygous truncating mutations. Interestingly, the previously unannotated Efcab3-like gene is very close to the 17q21.31 syntenic region associated in humans to the Koolende Vries microdeletion syndrome (KdVS). We used the presence of dense clusters of hSNPs on the C57BL/6J reference genome as a marker for extreme polymorphism and examined the de novo assembly to explore the underlying genomic architecture. OG Kush The Strains aroma is woodsy or piney, and pungent is the best way to describe it. A consensus-finding algorithm was employed to decide between possible versions of an orthologous transcript. 3a) and an Efcab3-like knockout, showed analogous brain phenotypes, suggesting common cis-acting regulatory mechanisms as shown previously in the context of the 16p11.2 microdeletion syndrome68. The genome structure of the Nlrp1 locus in PWK/PhJ, CAST/EiJ, WSB/EiJ, and NOD/ShiLtJ was confirmed using Fiber-FISH (Supplementary Fig. You are using a browser version with limited support for CSS. Chromosomal contacts connect loci associated with autism, BMI and head circumference phenotypes. Supplementary Figs. Mi, H., Muruganujan, A. Rev. The sensory results After ligation, cross-links were reversed and the DNA was purified from protein. Grey blocks indicate repeats and transposable elements. Appl Microbiol Biotechnol. 102, 23692378 (2007). We identified between 116,439 (C57BL/6NJ) and 1,895,741 (SPRET/EiJ) high-quality hSNPs from the MGP variation catalog v521 (Supplementary Table 9). SPRET/EiJ contributed the largest number of strain-specific gene hits (22 genes). 1c). Nat. Accessibility Novel coding sequence discovered in this study is shown in yellow. CBD (cannabidiol), on the other hand, is non-intoxicating and does not produce a psychoactive high. Cactus: Algorithms for genome multiple sequence alignment. Ye, Z. et al. Bookshelf This represented the density at which the interval content and total unique overlapping base pairs was observed to be clustered around distinct loci (Supplementary Fig. 38, 240244 (2006). At the novel bicistronic locus, Chml_Opn3, the original annotation was a single exon gene, Chml, that was extended and found to share its first exon with the Opn3 gene. is supported by R01 DK074656. The pontine nuclei were also increased in size by 42% (P=0.001) and the cerebellum by 27% (P=0.02); these two regions are involved in motor activity (Fig. Dummer, P. D. et al. Main Terpenes: Myrcene, Pinene and Limonene. Headspace can and transmitted securely. It helps stimulate an appetite in people who have trouble eating due to stress or depression. Novel members are named after their most similar homologs. Inbred laboratory mouse strains are characterized by at least 20 generations of inbreeding and are genetically homozygous at almost all loci1. In this application, two different types of evidence are used: the RNA-Seq hints for each of the novel strains from above; and annotation evidence from GENCODE VM8 for the C57BL/6J reference strain. evan peters jeffrey dahmer & Academic Background; department of public works massachusetts. d, A regional dot plot of the Nlrp1 locus in PWK/PhJ compared to the C57BL/6J GRCm38 reference (color-coded same as panel c). Terpenes are aromatic compounds found in cannabis and other plants that contribute to each strain's unique scent and flavor profile. There are currently 11 genes in the GRCm38 reference assembly (C57BL/6J) that are incomplete due to a gap in the sequence. Kelada, S. N. P. et al. Learn about safe consumption, the impact of legalization, and how you can join the festivities! Mouse models of 17q21.31 microdeletion and microduplication syndromes highlight the importance of Kansl1 for cognition. Fine mapping in 94 inbred mouse strains using a high-density haplotype resource. By accessing this site, you accept the Terms of Use and Privacy Policy. You must take responsibility for abiding the law of the city, country or the jurisdiction where you are located. Nat. Cosubstrate independent mineralization of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by a Desulfovibrio species under anaerobic conditions. Top autoflower seeds to grow for spring and summer 2023. All mice were maintained in a specific pathogen-free facility with sentinel monitoring at standard temperature (1923C) and humidity (55% 10%), on a 12 h dark, 12 h light cycle (lights on 7:3019:00) and fed a standard rodent chow (LabDiet 50213, 9% crude fat content, 21% kcal as fat, 0.276 ppm cholesterol). Google Scholar. P.F. carried out the genome analysis. Inbred laboratory strains of mice are broadly organized into two groups, classical and wild-derived strains1, that can be used to model the variation observed in human populations2,3. Terpenes may also influence the strain's effects and are believed to work synergistically with cannabinoids in what is known as the "entourage effect.". Curr. Laws vary by location, and it's important to ensure compliance. The regions identified in C57BL/6J and C57BL/6NJ (117 and 141, respectively; 145 combined) intersect known GRCm38 assembly issues including gaps, unplaced scaffolds, or centromeric regions (107/145, 73.8%). Nature 474, 337342 (2011). We assessed the base accuracy of the strain chromosomes relative to two versions of the C57BL/6J reference genome (MGSCv311 and GRCm382) by first realigning all of the paired-end sequencing reads from each strain back to their respective genome assemblies, then using these alignments to identify SNPs and indels. It is best used in small doses or as a pre-workout boost, so you can get the most out of your workout while still being able to function when it's time to head home. Boroviak, K., Doe, B., Banerjee, R., Yang, F. & Bradley, A. Chromosome engineering in zygotes with CRISPR/Cas9. Collaborative Cross mice and their power to map host susceptibility to Aspergillus fumigatus infection. Discover the true story behind 420, the iconic day celebrated by cannabis enthusiasts around the world. Nature 491, 125128 (2012). White coloring indicates a P value>0.05 and grey indicates that the brain region could not be confidently tested due to missing data. WebIts easy to grow even if this is your first-ever strain. WebFind information about the Headspace strain from Sunday Goods such as potency, common effects, and where to find it. & Thomas, P. D. PANTHER in 2013: modeling the evolution of gene function, and other gene attributes, in the context of phylogenetic trees. Pairs of flanking guide RNAs (gRNAs) were designed using the WTSI Genome Editing (WGE) tool78 creating four gRNAs (two gRNAs 5 and two gRNAs 3 to the CE region, Supplementary Table 21). Behav. WebThe goals for Headspace are threefold: (a) develop an initial olfactory lexicon for cannabis bud using standard sensory research methods; (b) determine whether there are In most cases where a new locus was predicted on the reference genome, we identified pre-existing, but often incomplete, annotation. The separations of Chicago read pairs mapped within draft scaffolds were analyzed by HiRise to produce a likelihood model for genomic distance between read pairs. Assembly scaffolds are further joined into chromosomes so as to minimize the number of structural differences (such as inversions or chromosomal translocations) between references and a target genome. Shotgun and Chicago library sequences were aligned to the draft input assembly using a modified SNAP read mapper (see URLs). To identify defense genes from the analysis shared among classical inbred strains and each of the wild-derived strains, each of the strain-specific gene sets were merged into five categories, namely classical inbred (BALB/cJ, CBA/J, DBA/2J, C3H/HeJ, 129S1/SvImJ, A/J, C57BL/6NJ, NOD/ShiLtJ, LP/J, NZO/HlLtJ, FVB/NJ and AKR/J), PWK/PhJ, CAST/EiJ, WSB/EiJ, and SPRET/EiJ (Fig. The care and use of mice in the study was carried out in accordance with UK Home Office regulations, UK Animals (Scientific Procedures) Act of 1986 under a UK Home Office license that approved this work, which was reviewed regularly by the WTSI Animal Welfare and Ethical Review Body. The transfer line flow rate, for the needle configuration, should be 50% of the column flow rate. Despite this, previous SNP variation catalogs have identified high-quality heterozygous SNPs (hSNPs) when reads were aligned to the C57BL/6J reference genome12. 10 feminised seedsActual special-offer onCannapot HanfshopNow 10.00 % reduced! Epub 2008 Sep 9. For the first time, the underlying sequence at these loci is represented in strain-specific assemblies and gene predictions from across the inbred mouse lineage, which should facilitate increased dissection of complex traits. B.P., I.T.F., M.A., J.A., M.Di., D.D.D., D.E., A.F., M.G., J.G., J.H., S.N., P.M., F.C.P.N., L.Ro., M.S., C.Si., C.St., G.T., R.B., J.Lo., M.S.H., D.T., and J.Li. Analysis of Genotype-Tissue Expression (GTEx) data62 in humans showed that the EFCAB13 locus is expressed across many tissue types, with the highest expression measured in testis and thyroid. ISSN 1061-4036 (print). We're still gathering information about the Headspace strain. 9, e1003196 (2013). Biodegradation of the nitramine explosives hexahydro-1,3,5-trinitro-1,3,5-triazine and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine in cold marine sediment under anaerobic and oligotrophic conditions. Ferris, M. T. et al. Cannabis strains are generally classified into three main types: Indica, Sativa, and Hybrid. Kirby, A. et al. However, reliance on mapping next-generation sequencing reads to C57BL/6J has meant that the true extent of strain-specific variation is unknown. This heady hybrid opens the mind and lays out new paths for thinking about yourself and those around you. Briefly, the constitutive coding exon 5 (chromosome 11: 104700610-104700692, GRCm38), which is well-supported by RNA-Seq data in multiple tissues (ENSMUST00000212287; ENSMUSE00000376310 (ENSEMBL v90)) was deleted using the SpCas9 endonuclease to induce a frameshift mutation. Recommendations: Use low shake. It goes without saying it is the The 16S rRNA gene sequence for strain HAAP-1, consisting of 1485 base pairs, After scaffolding, shotgun sequences were used to close gaps between contigs. Curing and trimming the buds can be something that takes you multiple days. Efcab3/Efcab13 encode calcium-binding proteins and the new gene primarily consists of repeated EF-hand protein domains (Supplementary Fig. Next, we used a set of 612 polymerase chain reaction (PCR) primer pairs previously used to validate structural variant calls in eight strains22. 2c), whereas in M. spretus (also wild-derived) this locus is much shorter than in any other mouse strain. Diversity Outbred mice identify population-based exposure thresholds and genetic factors that influence benzene-induced genotoxicity. 7). All DNA was obtained from the Jackson Laboratories from female mice (Supplementary Table 17). The assemblies had 4.76.7% primer pairs showing incorrect alignments compared to 10% for MGSCv3 (Supplementary Table 6). Discover 7 surprising ways to use hemp in your daily life, from beauty products to food. Approximately 0.52% of total genome length per strain was unplaced and is composed of unknown gap bases (1849%) and repeat sequences (6179%) (Supplementary Table 2), with between 89 and 410 predicted genes per strain (Supplementary Table 3). De novo genome assembly methods address this issue by allowing unbiased assessments of the differences between genomes. Strip the bolt and scrub it clean. 2a and Supplementary Table 13)42. a, Olfactory receptor genes on chromosome 11 of CAST/EiJ. 1e). volume50,pages 15741583 (2018)Cite this article. Evolutionary history of mammalian transposons determined by genome-wide defragmentation. The subsequent reads were used as input to SOAPdenovo273 r240 to produce genome scaffolds (parameters given in Supplementary Table 19). was supported by a research grant from CONICYT/FONDECYT/REGULAR No.1171004 and the European Commission (EUFP7 BLUEPRINT grant HEALTH-F5-2011-282510). 11 and Supplementary Data 7) and the near-complete inclusion of the Sts gene that was previously missing. On average, 10% of synteny block adjacencies in the assembled genomes were not presented in C57BL/6J reference.
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